primary human follicle dpcs  (PromoCell)

Journal: Heliyon

Article Title: Mycoplasma hyorhinis infection promotes TNF-α signaling and SMAC mimetic-mediated apoptosis in human prostate cancer

doi: 10.1016/j.heliyon.2023.e20655

Figure Lengend Snippet: M. hyorhinis promotes TNF-α secretion from PCa cells. (A), relative TNF-α secretion levels in M. hyorhinis-contaminated PCa cells (PC3-cM and C4–2B-cM) and mycoplasma-free cells; multiple cancer cells (PC3, C4–2B, DU145, LNCaP, MDA-MB-231, MCF-7, MCF10A, HeLa, MG-63), HDMEC, HF, and DPC via ELISA. (B), experimental design. The parental PCa cells (PC3–P and C4–2B–P) were infected with M. hyorhinis (3 × 107 CFU, 5 MOI) for 2 passages and then passaged twice a week for 6 weeks without further infection (PC3-M and C4–2B-M). For PC3-MF and C4–2B-MF cells, 4 weeks after infection, M. hyorhinis was eliminated in cell cultures for 3 weeks. Seven weeks after infection, in vitro assays and analyses were performed using these PCa cells. (C), relative TNF-α secretion levels in the parental (PC3–P and C4–2B–P) and M. hyorhinis-infected (PC3-M and C4–2B-M) PCa cells. (D), relative TNF-α secretion levels in PC3-M and C4–2B-M and previously infected PCa cells after elimination of M. hyorhinis (PC3-MF and C4–2B-MF). M. hyrorhinis infection was confirmed by Western blotting using specific anti-M. hyorhinis (P70 surface antigen) antibody. βactin was used as a loading control. See full images in Supplementary Figure S4. All results represent mean ± SD values from triplicate assays, and the experiments were repeated three times. **p < 0.0001. HDMEC, human dermal microvascular endothelial cells; HF, human primary fibroblasts; DPC, human primary dental pulp cells; CFU, colony-forming units; MOI, multiplicity of infection.

Article Snippet: Human primary fibroblasts (HF) [ ], human primary dental pulp cells (DPC) [ ], and human cancer cell lines (PC3, C4–2B, DU145, LNCaP, MDA-MB-231, MCF-7, MCF10A, HeLa, MG-63; American Type Culture Collection (ATCC), Manassas, VA) were grown in Dulbecco's modified Eagle's medium (DMEM, Invitrogen, Carsbad, CA) supplemented with 10 % FBS and 1 % penicillin/streptomycin.

Techniques: Enzyme-linked Immunosorbent Assay, Infection, In Vitro, Western Blot

Journal: Cell Transplantation

Article Title: Effect of Cinnamomum osmophloeum Kanehira Leaf Aqueous Extract on Dermal Papilla Cell Proliferation and Hair Growth

doi: 10.1177/0963689717741139

Figure Lengend Snippet: The Cinnamomum osmophloeum Kanehira extract enhances the proliferation of cultured rat vibrissae dermal papilla cells (DPCs). Cell proliferation was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Mino: minoxidil. Values are the mean ± SD . * P < 0.05 and ** P < 0.01, compared with untreated cells (control).

Article Snippet: Primary human hair DPCs (hDPCs) were purchased from ScienCell Research Laboratories (Carlsbad, CA, USA) as primary cells and maintained in mesenchymal stem cell (MSC) medium (ScienCell Research Laboratories), 5% (w/v) FBS, 1% (w/v) penicillin, 1% (w/v) mesangial cell growth supplement (ScienCell Research Laboratories) under a 5% CO , humidified environment at 37 °C.

Techniques: Cell Culture, MTT Assay, Control

Journal: Cell Transplantation

Article Title: Effect of Cinnamomum osmophloeum Kanehira Leaf Aqueous Extract on Dermal Papilla Cell Proliferation and Hair Growth

doi: 10.1177/0963689717741139

Figure Lengend Snippet: The Cinnamomum osmophloeum Kanehira extract enhances the proliferation of cultured human hair dermal papilla cells (hDPCs). Cell proliferation was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Mino: minoxidil. Values are the mean ± SD . * P < 0.05 and ** P < 0.01, compared with untreated cells (control).

Article Snippet: Primary human hair DPCs (hDPCs) were purchased from ScienCell Research Laboratories (Carlsbad, CA, USA) as primary cells and maintained in mesenchymal stem cell (MSC) medium (ScienCell Research Laboratories), 5% (w/v) FBS, 1% (w/v) penicillin, 1% (w/v) mesangial cell growth supplement (ScienCell Research Laboratories) under a 5% CO , humidified environment at 37 °C.

Techniques: Cell Culture, MTT Assay, Control

Journal: Cell Transplantation

Article Title: Effect of Cinnamomum osmophloeum Kanehira Leaf Aqueous Extract on Dermal Papilla Cell Proliferation and Hair Growth

doi: 10.1177/0963689717741139

Figure Lengend Snippet: Quantitative polymerase chain reaction of growth factor genes in human hair dermal papilla cells (hDPCs). Changes in growth factor messenger RNA (mRNA) expression levels induced after different treatments for 48 h. The growth factor mRNA expression levels were normalized to that of β-actin mRNA expression with the results expressed as fold changes.

Article Snippet: Primary human hair DPCs (hDPCs) were purchased from ScienCell Research Laboratories (Carlsbad, CA, USA) as primary cells and maintained in mesenchymal stem cell (MSC) medium (ScienCell Research Laboratories), 5% (w/v) FBS, 1% (w/v) penicillin, 1% (w/v) mesangial cell growth supplement (ScienCell Research Laboratories) under a 5% CO , humidified environment at 37 °C.

Techniques: Real-time Polymerase Chain Reaction, Expressing

Journal: Cell Transplantation

Article Title: Effect of Cinnamomum osmophloeum Kanehira Leaf Aqueous Extract on Dermal Papilla Cell Proliferation and Hair Growth

doi: 10.1177/0963689717741139

Figure Lengend Snippet: Effect of Cinnamomum osmophloeum Kanehira (COK) extract and minoxidil on the hair follicle unit area and skin thickness of mice. (a) Biopsied dorsal skin of C57BL/6 mice at days 1 and 14 was fixed and then stained with hematoxylin and eosin. Effects of topical COK extracts on C57BL/6 mouse dermal papilla cells (DPCs) during the anagen phase induction shown by representative skin samples from 4 animals. (b) Quantified slides from these samples. Scale bars = 200 µm.

Article Snippet: Primary human hair DPCs (hDPCs) were purchased from ScienCell Research Laboratories (Carlsbad, CA, USA) as primary cells and maintained in mesenchymal stem cell (MSC) medium (ScienCell Research Laboratories), 5% (w/v) FBS, 1% (w/v) penicillin, 1% (w/v) mesangial cell growth supplement (ScienCell Research Laboratories) under a 5% CO , humidified environment at 37 °C.

Techniques: Staining